壮药藤苦参中强心苷的含量测定及不同产地、采收季节对药材含量的影响

　　[摘要]目的 建立壯药材藤苦参中强心苷的含量测定方法，研究不同产地、采收季节对药材含量的影响。方法 利用甲型强心苷α，β-不饱和内酯环的显色反应，采用紫外分光光度法建立藤苦参中强心苷含量测定的方法，并进行方法学考察，测定不同产地、采收季节的藤苦参药材中强心苷含量。结果 按紫外分光光度法，以地高辛为对照品，碱性苦味酸溶液为显色剂，415 nm的波长下检测，在0～0.08 mg/ml范围内对照品含量与吸光度呈良好的线性关系；回归方程为y=14.968x+0.008（r=0.9969）；精密度RSD=0.1%；平均加样回收率为95.51%，RSD为2.16%（n=6）。测定6批藤苦参中强心苷平均含量，为2.07 mg/g。测定广西四个不同产地及不同采收季节藤苦参强心苷含量，结果产于钦州八塞沟、并于10～12月采收的藤苦参中强心苷含量最高。结论 所建立的藤苦参强心苷的含量测定方法，稳定性和重复性高，回收试验良好，可作为藤苦参药材的质量控制方法使用。不同产地及不同采收季节对藤苦参强心苷含量有显著影响。
　　[关键词]藤苦参；强心苷；紫外分光光度法；产地；采收季节
　　[中图分类号] R927.2 [文献标识码] A [文章编号] 1674-4721（2018）4（c）-0014-04
　　Determination of the content of cardiac glycoside in Zhuang herb Streptocaulon griffithii and its influencing factors of different producing areas and harvesting seasons
　　LUO Yu-dong1，2，3 TAN An-qiang1▲ LU Shi-heng1 WU Yu-qiang1，3 ZHANG Qiang1
　　1.Pharmaceutical Factory，Guangxi University of Chinese Medicine，Nanning 530023，China；2.Key Laboratory of Zhuang Medical Formulae and Applied Research of Colleges and Universities in Guangxi Zhuang Autonomous Region，Nanning 530001，China；3.Key Laboratory of Common Technology Research and Development of Traditional Chinese Medicine Preparation of Colleges and Universities in Guangxi Zhuang Autonomous Region，Nanning 530001，China
　　[Abstract]Objective To establish a method for the determination of cardiac glycoside in Zhuang herb Streptocaulon griffithii，and to study the influence of different producing areas and harvesting seasons on the content of medicinal herb.Methods The colorimetric reaction of α，β-unsaturated lactone ring of type A cardiac glycosides was used to establish the method for the determination of the content of cardiac glycoside in Streptocaulon griffithii by ultraviolet spectrophotometry （UV spectrophotometry）.The methodology was also verified.The content of cardiac glycoside in Zhuang herb Streptocaulon griffithii from different producing areas and harvesting seasons was determined.Results By UV spectrophotometry，Digoxin was used as reference substance，and alkaline picric acid solution was used as chromogenic agent.The good linear relation of the content of reference substance and absorbance was displayed in the range of 0-0.08 mg/ml in 415 nm wavelength.The regression equation y=14.968x+0.008 （r=0.9969）.The precision of RSD was 0.1% and average recovery was 95.51% （n=6；RSD=2.16%）.The average content of cardiac glycosides from 6 batches of Streptocaulon griffithii was 2.07 mg/g.The cardiac glycosides of Streptocaulon griffithii from four different producing areas and seasons in the Guangxi Zhuang Autonomous Region were determined.The highest content of cardiac glycoside of Streptocaulon griffithii was from Basaigou，Qinzhou City and was collected from October to December yearly.Conclusion The established method for the determination of cardiac glycosides of Streptocaulon griffithii has high stability and repeatability in good recovery，and can be used as quality control for Streptocaulon griffithii.Different producing areas and harvesting seasons have significant impact on the content of cardiac glycosides of Streptocaulon griffithii.

相关热词搜索：含量 强心 苦参 采收 测定